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从小麦ＣＫ８９、１０２、１１３的花药和花冬的约穗诱导愈伤组织的继代培养中，选择致密颗粒型愈伤组织，转入附加２，４－Ｄ２～４ｍｇ／Ｌ改良ＭＳ固体培养基上进行继代培养，６～１０周后形成生长迅速的胚性愈伤组织，这种愈伤组织分离原生质体得率为１～３．７×１０７个／ｇ。原生质体于ＰＣＭ２培养基上琼脂糖包埋培养，３～５天出现第一次细胞分裂，７～１４天进行第二、第三次细胞分裂，两周后形成大量细胞团，细胞分裂频率达２０．３％～７３．３％，４～６周后形成肉眼可见愈伤组织。供试４个基因型中，ＣＫ８９、１０２和花冬均形成再生愈伤组织，仅１１３形成多细胞团。愈伤组织增殖分化实验尚在进行中。
From subculture of callus induced from spikelets of anthers and anther of wheat CK89, 102, 113, the compact callus was selected and transferred into an additional MS medium with 2,4-D2 ~ 4mg / L The embryogenic callus grew rapidly after 6 ~ 10 weeks. The protoplast yield of this callus was 1 ~ 3.7 × 107 cells / g. Protoplasts were cultured on PCM2 medium with agarose, the first cell division occurred in 3-5 days, the second and third cell division occurred in 7-14 days, and a large number of cell masses formed after two weeks. The frequency of cell division reached 20.3% ~ 73.3%, 4 ~ 6 weeks after the formation of visible callus. Among the four genotypes tested, regenerated callus was formed in both CK89, 102, and Hua Dong, with only 113 forming multicellular clusters. Callus proliferation and differentiation experiments are still underway.