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目的:近年来许多报道表明,miRNA与一些肿瘤的发病息息相关,其其表达失调直接或间接的影响肿瘤的进展。检测mir-26a在子宫肌瘤组织和肌层组织中的表达,并进一步探究其对子宫肌瘤细胞周期和增殖的影响。方法:收集第二军医大学长海医院妇产科2009年12月至2011年10月手术治疗并经病理检查确诊为子宫肌瘤的患者肌瘤组织和成对肌层组织标本13例,Realtime PCR检测这13例成对组织中mir-26a的表达情况。建立可稳定传代的子宫肌瘤平滑肌细胞系后,将mir-26a转入肌瘤细胞中使其高表达,流式细胞仪检测高表达mir-26a后子宫肌瘤细胞周期的变化,同时用cck-8方法验证细胞增殖活性。结果:Realtime PCR结果显示,与相应肌层组织相比,肌瘤组织mir-26a的表达均显著降低(P<0.05)。流式检测结果显示,24 h时,与对照组相比,mir-26a高表达的肌瘤细胞G1期比例增高。增殖实验显示种植细胞第二天开始,mir-26a高表达的子宫肌瘤细胞增殖速度显著降低。结论:与正常子宫肌层组织相比,mir-26a在子宫肌瘤组织中表达下调,这种表达失调直接影响肌瘤细胞的增殖速度和周期比例,提示mir-26a在子宫肌瘤的发生发展过程中发挥了重要的调控作用。
OBJECTIVE: In recent years, many reports indicate that miRNA is closely related to the pathogenesis of some tumors, and the dysregulation of its expression directly or indirectly affects tumor progression. The expression of mir-26a in uterine fibroids and myometrial tissues was detected and the effect on the cell cycle and proliferation of uterine fibroids was further explored. Methods: Thirteen cases of myoma and paired myometrium were collected from the Department of Obstetrics and Gynecology, Changhai Hospital, Second Military Medical University from December 2009 to October 2011 and were diagnosed as uterine myoma by pathological examination. Realtime PCR The 13 cases of paired tissue mir-26a expression. After establishment of stable passage myoma of uterine fibroids smooth muscle cell line, the mir-26a into the fibroids to make it high expression, flow cytometry detection of mir-26a high expression of uterine fibroids cell cycle changes, while cck -8 method to verify cell proliferation activity. Results: The results of Realtime PCR showed that the expression of mir-26a in fibroids was significantly lower than that in corresponding muscular tissues (P <0.05). The results of flow cytometry showed that the proportion of G1 phase of mir-26a-overexpressed fibroids increased at 24 h compared with the control group. Proliferation experiments showed that the proliferation of mir-26a-overexpressed uterine fibroids was significantly reduced at the second day after implantation. Conclusion: Compared with normal myometrium, mir-26a is down-regulated in uterine fibroids, which directly affects the proliferation rate and cycle proportion of fibroids, suggesting the development of mir-26a in uterine fibroids The process played an important role in regulation.