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研究利用高效液相色谱法研究了强力霉素在斑点叉尾(Ictalurus punctatus)体内的药物动力学与消除规律,有助于制定合理用药方案和休药期,为水产品质量安全提供理论依据。(1)单次口服剂量20 mg/kg强力霉素在斑点叉尾体内的药时数据符合二室开放式模型。药-时曲线呈明显双峰现象:第一次达峰时,强力霉素在肾、血和肌肉中浓度迅速上升,达峰时间Tmax(1)出现在30min,强力霉素在肝脏中浓度上升缓慢,出现在1h;肝、肾、血和肌肉第二次达峰的时间Tmax(2)出现在8h,第二次达峰浓度Cmax(2)大于第一次的浓度Cmax(1)。药-时曲线下面积(AUC):肾、肝、血和肌肉分别为63.242、1282.077、142.379、62.348μg.h/mL。消除半衰期[T1/2b]:肾、肝、血和肌肉分别为40.668、48.767、36.527、31.091h,平均滞留时间(MRT):肾、肝、血和肌肉分别为46.585、56.989、48.859、42.428h;(2)连续口服剂量20 mg/kg的强力霉素5d,停药后强力霉素在斑点叉尾肝脏中浓度最高,肌肉+皮中浓度最低。在不同组织中强力霉素的消除速率不同(P<0.05),药物消除速度由高到低依次为肌肉+皮、肾脏、肝脏。若以肝脏为靶组织,最高残留限量300μg/kg,休药期不低于30d;若以可食组织肌肉+皮为靶组织,最高残留限量300μg/kg,休药期不低于19d。
In this study, we investigated the pharmacokinetics and elimination of doxycycline in Ictalurus punctatus using high performance liquid chromatography (HPLC), which is helpful for the formulation of rational drug regimens and withdrawal periods and provide a theoretical basis for the quality and safety of aquatic products . (1) The single oral dose of 20 mg / kg doxycycline in the channel catfish drug data consistent with two-compartment open model. Drug-time curve showed a bimodal phenomenon: the first peak, the concentration of doxycycline in the kidney, blood and muscle increased rapidly, peak time Tmax (1) appeared in 30min, doxycycline increased in the liver concentration Tmax (2) appeared at 8h, the second peak concentration Cmax (2) was greater than the first concentration Cmax (1). Pharmacokinetic-area under the curve (AUC): kidney, liver, blood and muscle were 63.242,1282.077,142.379,62.348μg.h / mL. Elimination half-life [T1 / 2b]: kidney, liver, blood and muscle were 40.668,48.767,36.527,31.091h, mean retention time (MRT): kidney, liver, blood and muscle were 46.585,56.989,48.859,42.428h ; (2) Continuous oral administration of 20 mg / kg of doxycycline for 5 days resulted in the highest concentration of doxycycline in the liver of the channel catfish, with the lowest concentration in muscle and skin. In different tissues, the elimination rate of doxycycline was different (P <0.05). The order of elimination of drug was muscle + skin, kidney and liver in descending order. If the liver as the target tissue, the maximum residue limit of 300μg / kg, withdrawal period of not less than 30d; if edible tissue muscle + skin as the target tissue, the maximum residue limit 300μg / kg, withdrawal period of not less than 19d.