本文将含ＨＰＶ重组质粒的大肠杆菌活化、扩增、提取、酶切回收，获得纯化的７．９ｋｂＨＰＶＤＮＡ片段，通过随机引物标记法制成Ｄｉｇ－ＨＰＶＤＮＡ探针。用此探针进行斑点杂交，Ｓｏｕｔｈｅｒｎ转印杂交检测女性生殖器活检组织标本中的ＨＰＶＤＮＡ，并检测其敏感性、特异性及重复性。１３３例活检组织标本中，经病理切片诊断为尖锐湿疣的标本，其ＨＰＶ６ｂ和ＨＰＶ１１检出率为８０．６５％，非尖锐湿疣为６．９３％。试验证明，此探针检测ＨＰＶＤＮＡ特异性强、敏感性高、背景清晰、有效期长，适用于临床检测。 In this paper, the E.coli containing HPV recombinant plasmids was activated, amplified, extracted and digested. The purified 7.9kb HPP DNA fragment was obtained and the Dig-HPVDNA probe was made by random primer labeling. Dot blot hybridization was performed with this probe. Southern blotting was used to detect HPVDNA in female genital biopsies and its sensitivity, specificity and repeatability were tested. In 133 biopsy specimens, the specimens diagnosed as condyloma acuminatum by pathology showed 80.65% of HPV6b and HPV11, and 6.93% of non-condyloma acuminatum. Test proved that this probe to detect HPVDNA specific, high sensitivity, clear background, long validity, suitable for clinical testing.