采用ＰＲＡＳＴＡＴ技术筛选肾移植受者特异性抗ＨＬＡ抗体（ｓＨＬＡＩｇＧ）。将１２８份血清分成４组，包括首次移植术前及术后一个月、肾功能稳定期和移植肾失功再血透者。按ＰＲＡＳＴＡＴ酶联检测试剂盒要求操作。抗体特异性的确定根据阳性结果的反应格局综合判断。结果表明所有样本的测定结果其有效性均符合试剂盒要求。本法操作简捷、耗时４ｈ；结果重复性好、批内与批间差异（ＣＶ）均＜５％；ＰＲＡＳＴＡＴ百分比＜８０％的致敏者其抗体特异性可以确定。临床应用显示：再次移植者致敏率高达７５％，显著高于首次移植者（１８８％）；术前及术后ｓＨＬＡＩｇＧ抗体的监测对移植效果具有预测和指导意义。 PRA  STAT screening of renal transplant recipients specific anti-HLA antibodies (sHLA Ig). 128 serums were divided into 4 groups, including the first transplantation and one month after surgery, stable renal function and transplanted renal failure and hemodialysis. Press PRA  STAT enzyme detection kit required operation. The specificity of the antibody is determined based on the reaction pattern of the positive result. The results showed that all the samples were validated in accordance with the requirements of the kit. This method is simple and time-consuming, 4h; results reproducible, intra-and intra-batch differences (CV) were less than 5%; PRA  STAT percentage <80% of the allergy to its antibody specificity can be determined. The clinical application shows that the sensitization rate of retransplantation is as high as 75%, which is significantly higher than that of first transplant (188%). The monitoring of sHLAIgG antibody before and after surgery has predictive and instructive significance for transplantation.