[Objective]To study the optimum extraction technology condition of ultrasonic-assisted semi-bionic extraction of total flavonoids from Soutellaria barbata. [Methods]To explore the effects of ultrasonic power,extraction time,liquid ratio,extraction temperature and ethanol concentration on extraction ratio of total flavonoids by single factor test method and to optimize the extraction process. [Results]The optimum extraction technology conditions: the ultrasonic power was 60 W,the extraction time was 30 min,the liquid ratio was 1∶30( g / mL),the ethanol concentration was 60%,the extraction temperature was 60 ℃. And the extraction ratio of total flavonoids reached 3. 01% under these conditions. [Conclusions] Ultrasonic-assisted semi-bionic was suitable for extracting total flavonoids from Sculellaria barbata. [Objective] To study the optimum extraction technology condition of ultrasonic-assisted semi-bionic extraction of total flavonoids from Soutellaria barbata. [Methods] To explore the effects of ultrasonic power, extraction time, liquid ratio, extraction temperature and ethanol concentration on extraction ratio of total flavonoids by single factor test method and to optimize the extraction process. [Results] The optimum extraction technology conditions: the ultrasonic power was 60 W, the extraction time was 30 min, the liquid ratio was 1:30 (g / mL) , and the extraction temperature was 60 ° C. And the extraction ratio of total flavonoids reached 3.1% under these conditions. [Conclusions] Ultrasonic-assisted semi-bionic was suitable for extracting total flavonoids from Sculellaria barbata.