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目的评价发光二极管荧光染色法查找痰中抗酸分枝杆菌的效果。方法收集疑似肺结核患者1 026份痰标本分别进行发光二极管荧光染色法和萋-尼氏抗酸染色法检测,比较2种方法检测抗酸分枝杆菌的镜检时间和阳性检出率等方面的差异。结果发光二极管荧光染色法比萋-尼氏抗酸染色法的镜检时间明显缩短,差异具有统计学意义(P<0.05);发光二极管荧光染色法的阳性率明显高于萋-尼氏抗酸染色法,差异具有统计学意义(P<0.05)。结论发光二极管荧光染色法比萋-尼氏抗酸染色法的阳性率明显提高、镜检时间明显缩短等优点,是较为理想的结核病细菌学检测方法,更适合于基层实验室开展。
Objective To evaluate the effect of fluorescent staining of light-emitting diodes on the identification of Mycobacterium acid-fast acid in sputum. Methods A total of 1026 sputum specimens from patients with suspected pulmonary tuberculosis were collected by fluorescent staining with fluorescence-activated carbon (LED) staining and 萋-Nissl acid-fast staining, respectively. Two methods were compared to detect the time and the positive detection rate of Mycobacterium acidophilum difference. Results The microscopic examination time of LED fluorescent staining method was significantly shorter than that of 萋-Nisshore acid-fast staining method (P <0.05). The positive rate of LED fluorescence staining was significantly higher than that of 萋-Nissl acid Staining method, the difference was statistically significant (P <0.05). Conclusions The positive rate of fluorescent staining of Biot-Nismann blotting method is significantly higher than that of the fluorescence microscopy. The microscopic examination time is obviously shortened. It is an ideal bacteriological test method for tuberculosis and is more suitable for primary laboratory.