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~(13)C-尿素试验是特异性诊断人体内幽门螺旋杆菌(HP)感染的方法。此法为美国DY Graham和klein博士首创(1982)。后逐步应用于临床,它可以简便、快速、无痛苦和可靠地筛选人群的HP感染.是目前临床检查HP感染和根除治疗后疗效观察的最佳方法,其敏感度和特异度均可达到90％以上。 材料和方法:①检测对象:受检者经纤维胃镜检查,于胃窦部钳取标本组织细菌培养,HP阳性者为~(13)C-尿素呼气试验对象,共30例。②试剂、器材及方法:同位素比值质谱仪(GIRNS)由北京踏实科贸有限公司提供,~(13)C-尿素75mg由北京嘉汇彬医药技术有限公司提供。受检者于空腹状态下(通常要求空腹过夜或者空腹达418小时以上)进行,并在整个检查过程中保持安静(坐、卧均可)状态。受检者先空腹呼气,收集
~ (13) C-urea test is a method of specifically diagnosing Helicobacter pylori (HP) infection in the human body. This method was pioneered by the United States DY Graham and Dr. Klein (1982). And then gradually applied to clinical, it can be simple, rapid, painless and reliable screening of HP infection in the crowd is currently the best way to observe the clinical efficacy of HP infection and eradication therapy, the sensitivity and specificity can reach 90 %the above. MATERIALS AND METHODS: Subjects: Subjects were examined by fiberofiberoscopy, and specimens of gastric mucosa were dissected from the antral parenchyma. The subjects with HP positive were ~ (13) C-urea breath test, a total of 30 cases. ② reagents, equipment and methods: isotope ratio mass spectrometer (GIRNS) provided by the Beijing practical Electronics Co., Ltd., ~ (13) C-urea 75mg Beijing Jia Hui Bin Pharmaceutical Technology Co., Ltd. provided. Subjects under fasting conditions (usually require an overnight fasting or fasting for 418 hours or more), and remain quiet throughout the examination (sitting, lying may be) state. Subjects fasting breath, collect