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目的:维生素D受体(vitaminDreceptor,VDR)基因多态性已成为骨质疏松遗传学研究的热点,了解VDR基因多态性在中国老年男性中的分布,并进一步认识其与骨钙素及双羟维生素D3犤1,25(OH)2D3犦等的关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,分析145例老年男性的VDR基因型,同时采用放免法测定血清骨钙素(bonegla-containingprotein,BGP)、I型胶原前胶原氨基端前肽(N-terminalpropeptideoftypeprocollagen,PINP)及1,25(OH)2D3。结果:VDR基因型分布频率为BB,0.014;Bb,0.117;bb,0.869。B,b等位基因分别占7.2%,92.8%。骨质疏松症的发生率为38.6%。骨质疏松组与非骨质疏松组之间VDR基因型分布频率的差异无显著性意义(P>0.05)。未发现VDR基因型与BGP,PINP及1,25(OH)2D3相关。结论:老年男性VDR基因型与骨钙素等骨转换升华指标及1,25(OH)2D3无明显联系。
OBJECTIVE: The gene polymorphism of vitamin D receptor (VDR) has become a hotspot in the genetics of osteoporosis. To understand the distribution of VDR gene polymorphism in Chinese elderly men and to further understand its relationship with osteocalcin and bis Hydroxyvitamin D3 犤 1,25 (OH) 2D3 犦 and so on. Methods: PCR-RFLP was used to analyze the genotypes of VDR in 145 elderly men. The levels of serum osteocalcin (BGP), I N-terminal propeptide of typeprocollagen (PINP) and 1,25 (OH) 2D3. Results: The frequencies of VDR genotypes were BB, 0.014; Bb, 0.117; bb, 0.869. B, b allele accounted for 7.2%, 92.8%. The incidence of osteoporosis was 38.6%. There was no significant difference in the frequencies of VDR genotype between osteoporosis group and non-osteoporosis group (P> 0.05). No VDR genotype was found to be associated with BGP, PINP and 1,25 (OH) 2D3. Conclusion: VDR genotypes of elderly men and osteocalcin bone turnover sublimation indicators and 1,25 (OH) 2D3 no significant relationship.