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为了揭示BrFLC5对白菜类作物抽薹开花的作用机制,克隆了12份开花习性不同的白菜类作物BrFLC5基因exon2~exon4特异序列,分析发现在exon3的Pi3+1存在G–A变异,并开发了dCAPs标记。通过对来自10个不同栽培种群的93份材料进行开花时间调查和基因型检测,发现该基因的分子标记与开花时间表型显著相关(P<0.05)。因此,用该标记可以进行抽薹开花分子标记辅助选择育种。
In order to reveal the mechanism of BrFLC5 on bolting and flowering of Chinese cabbage crops, 12 exon2 ~ exon4 specific sequences of BrFLC5 gene were cloned in different flowering cabbage plants. It was found that there was G-A mutation in Pi3 + 1 of exon3 and dCAPs mark. Flowering time and genotypic analysis of 93 materials from 10 different cultivated populations showed that the molecular markers of this gene were significantly correlated with the flowering time phenotype (P <0.05). Therefore, bolting and flowering molecular marker assisted selection breeding can be carried out with this marker.