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本文研究了BrdU取代胸苷、紫外线照射染色体标本、5-氮咆苷去甲基化和染色体DNA盐酸变性对限制酶显带效应的影响。结果表明:1.BrdU取代胸苷不影响限制酶HaeⅢ和HinfⅠ的显带效应;2.限制酶HaeⅢ和PstⅠ不再使紫外线照射后的染色体标本显带;3.DNA去甲基化对限制酶HaeⅢ.HinfⅠ的显带效应无明显影响,但可使原先不能使染色体显带的HpaⅡ显示G或弱C带;4.染色体DNA盐酸变性不影响限制酶HaeⅢ和HinfⅠ的作用。
In this paper, the effects of BrdU substituted for thymidine, ultraviolet radiation on chromosomes, demethylation of 5-aza-glycosides and degeneration of chromosomal DNA in the presence of restriction enzymes were studied. The results showed that: 1. BrdU-substituted thymidine did not affect the banding effect of the restriction enzymes HaeIII and HinfI; 2. The restriction enzymes HaeIII and PstI no longer showed chromosomal banding after UV irradiation; 3. DNA demethylation on restriction enzymes The dominance effect of HaeIII.HinfI had no significant effect, but HpaII could not display G or weak C band. Chromosomal DNA degeneration did not affect the effects of the restriction enzymes HaeIII and HinfI.