用作者改进的液体培养基在5%CO_2环境中培养霍乱弧菌(Inaba 569B),每ml培养滤液可获得霍乱肠毒素(CT)1.215～2.012μg。在分离提纯CT时,先用DEAE-Sephadex A_(50)除去大部分色素及杂蛋白,再用Sephacryl S_(-200)分纯,效果好,重复性强;其第Ⅰ组分即为CT,肠襻试验阳性,双向琼脂扩散试验及HPLC检测结果均与上海CT一致。分子量为86000。 Vibrio cholerae (Inaba 569B) was cultured in a 5% CO 2 environment using the author’s modified liquid culture medium. From the culture filtrate, 1.215 to 2.012 μg of cholera enterotoxin (CT) was obtained. When separating and purifying the CT, first use DEAE-Sephadex A_(50) to remove most of the pigments and miscellaneous proteins, and use Sephacryl S_(-200) to separate them. The effect is good and the repeatability is strong; the first component is CT. Positive intestinal fistula test, two-way agar diffusion test and HPLC test results are consistent with Shanghai CT. The molecular weight is 86000.