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微核测定法自始建以来,已逐渐被广泛应用于快速评定理化诱变因子在活体内诱发染色体损伤的研究上。本文旨在应用长春新碱诱发体外长期培养细胞株的微核率,试图建立体外培养细胞株微核测定新体系。结果当一定浓度的长春新碱作用于体外培养的细胞株时,能够诱发其微核率升高,最高可达66‰(长春新碱的浓度为0.2微克/5毫升时),说明微核在体外也可诱发。也进一步证实了微核可以作为细胞受致突变物损害的生物学指征之一的论断。本文还对有关问题作了初步分析。
Since its establishment, micronucleus assay has been widely used in the rapid assessment of physicochemical mutagenesis induced chromosome damage in vivo. This article aims to use vincristine to induce the micronuclear rate of long-term cultured cell lines in vitro and attempt to establish a new micronucleus assay system for cultured cell lines in vitro. Results When a certain concentration of vincristine was applied to the in vitro cultured cell line, the micronucleus rate could be induced to rise up to 66 ‰ (when the concentration of vincristine was 0.2 μg/5 ml), indicating that the micronucleus was It can also be induced in vitro. It is further confirmed that micronucleus can be used as one of the biological indicators that cells are damaged by mutagens. This article also made a preliminary analysis of relevant issues.