论文部分内容阅读
考察了在大肠杆菌中自诱导表达人胰高血糖素样肽-1突变体融合蛋白的可行性,并对自诱导培养条件及培养基成分进行优化,以提高蛋白产量。实验结果表明,最优培养基成分为蛋白胨19.17g/L,酵母膏9.59g/L,Na2HPO45.72g/L,KH2PO45.48g/L,(NH4)2SO42.66g/L,NaCl3.33g/L,甘油2%(V/V),葡萄糖0.68g/L,乳糖6.33g/L,MgSO40.24g/L。在温度33°C、接种量1%、pH7、装瓶量20mL/100mL培养条件下,用该最优培养基自诱导表达人胰高血糖素样肽-1突变体融合蛋白的产量可达348.6mg/L。
The feasibility of self-induced expression of human glucagon-like peptide-1 mutant fusion protein in Escherichia coli was investigated, and self-induced culture conditions and media components were optimized to increase protein yield. The results showed that the optimal medium was 19.17 g / L of peptone, 9.59 g / L of yeast extract, 45.72 g / L of Na2HPO4, 45.48 g / L of KH2PO4, 42.66 g / L of (NH4) Glycerol 2% (V / V), glucose 0.68 g / L, lactose 6.33 g / L, MgSO 40.24 g / L. Under the conditions of 33 ° C temperature, 1% inoculation volume, pH7, and 20 mL / 100mL of bottling volume, the yield of fusion protein of human glucagon-like peptide-1 mutant self-induced by this optimal medium was up to 348.6 mg / L.