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采用逆转录及巢式聚合酶链式反应(RTPCR)和Southern杂交方法对肝癌患者的早期血行微转移灶进行检测并做临床随访观察。10例原发性肝癌患者(HCC),经手术切除的癌组织,检测AFPmRNA均为阳性。39例HCC患者中有24例外周血检测为阳性,阳性率61.5%。1a时间随访到28例患者,9例阴性结果的病人,1例死亡,死亡率为11.0%。3例临床证实有转移灶。19例阳性结果的病人,9例死亡,死亡率为47.3%,4例临床证实有转移灶。RTPCR结果不同的两组经卡方检验,P<0.01,患者预后也有明显的差异。检测HCC患者外周血中癌细胞是阳性结果,可以说明癌细胞已进入血循环。随访结果显示HCC患者外周血中AFPmRNA检测呈阳性则同患者出现血行微转移灶及预后有一定的相关性,可以给临床提供一个肿瘤有远处转移的参考指标。
Reverse transcription and nested polymerase chain reaction (RT-PCR) and Southern hybridization were used to detect early hepatic micrometastasis in patients with hepatocellular carcinoma. In 10 patients with primary liver cancer (HCC), surgically resected cancer tissues were tested positive for AFP mRNA. Of the 39 patients with HCC, 24 were found to be positive in peripheral blood, with a positive rate of 61.5%. At the time of 1a, 28 patients were followed up. In 9 patients with negative results, 1 patient died. The mortality rate was 11.0%. Three cases confirmed metastases. Of the 19 patients with positive results, 9 died, the mortality rate was 47.3%, and 4 patients had clinically confirmed metastatic lesions. The two groups with different RT-PCR results were tested by chi-square test (P<0.01). The prognosis of patients was also significantly different. Detection of cancer cells in peripheral blood of patients with HCC is a positive result, indicating that cancer cells have entered the blood circulation. Follow-up results showed that the detection of AFP mRNA in peripheral blood of patients with HCC was positive with the occurrence of micrometastasis in the blood and prognosis, and it could provide a reference for distant metastasis of the tumor.