本文用ANAE法对石腊包埋的胃肠组织及淋巴结进行染色,T淋巴细胞浆内出现1至数个红棕色斑点,单核细胞、上皮细胞和胃肠腺细胞为弥做件着色,B淋巴细胞基本阴性,证实此法可用于组织内T细胞测定,方法简便,便于基层推广运用。大致正常胃粘膜中T淋巴细胞占9.8％左右,胃体胃窦无明显区别,分布在上皮间及固有层。固有层T细胞多沿上皮层分布,可能与接受抗原刺激有关。 Paraffin-embedded gastro-intestinal tissues and lymph nodes were stained by ANAE method. There were 1 to several reddish-brown spots in the T-lymphocyte plasma. Mononuclear cells, epithelial cells, and gastrointestinal gland cells were color-coded and B lymphocytes were stained. The cells were basically negative, confirming that this method can be used for the determination of T cells in tissues. The method is simple and convenient for grassroots promotion. About 9.8% of normal gastric mucosa T lymphocytes, no significant difference between the stomach gastric antrum, distributed in the epithelial and lamina propria. The lamina propria T cells are mostly distributed along the epithelial layer and may be involved in antigen stimulation.