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目的 研究霉酚酸酯(MMF)在单侧输尿管梗阻(UUO)大鼠模型中,能否减少肾小管间质肌成纤维细胞(MyoF)的浸润、增殖及Ⅰ、Ⅲ型胶原(Col Ⅰ、Col Ⅲ)的沉积。方法 将54只大鼠中的36只行左输尿管结扎术,另外18只行假手术。结扎后的大鼠分为模型组和MMF组。术后第5、10及15天分别处死各组中的6只大鼠,用免疫组化方法测定增殖细胞核抗原(PCNA)、α-平滑肌肌动蛋白(α-SMA)及Col Ⅰ、Col Ⅲ的表达情况。行HE和Masson染色,动态观察肾脏病理学变化。结果 MMF能显著减少肾组织处于增殖状态下的细胞数目和肾小管间质区MyoF的浸润,减轻了Col Ⅰ、Col Ⅲ的沉积,并有效改善了肾脏的病理学改变。结论 MMF可减少输尿管梗阻后MyoF的浸润并抑制其增殖,从而改善UUO所致的肾脏损伤,提示MMF有潜在的延缓慢性肾功能衰竭进程的作用。
Objective To investigate whether mycophenolate mofetil (MMF) can reduce the infiltration and proliferation of tubulointerstitial myofibroblasts (MyoF) in rats with unilateral ureteral obstruction (UUO) Col III). Methods Thirty-six of the 54 rats were left ureter ligation, and the other 18 were sham-operated. Rats after ligation were divided into model group and MMF group. Six rats in each group were sacrificed on the 5th, 10th and 15th day after operation. The expressions of proliferating cell nuclear antigen (PCNA), α-smooth muscle actin (α-SMA) and Col Ⅰ, Col Ⅲ The expression of the situation. HE and Masson staining, dynamic observation of renal pathological changes. Results MMF could significantly reduce the number of proliferating kidney cells and the infiltration of MyoF in the tubulointerstitium, reduced the deposition of Col Ⅰ and Col Ⅲ, and effectively improved the renal pathological changes. Conclusion MMF can reduce MyoF infiltration and inhibit its proliferation after ureteral obstruction, thereby improving renal injury caused by UUO, suggesting that MMF can potentially delay the progression of chronic renal failure.