论文部分内容阅读
目的通过建立洋常春藤及其栽培品种中皂苷类化合物的HPLC-UV指纹图谱,采用聚类分析和主成分分析方法进行统计学分析研究,同时结合标定化合物的测定,以期为常春藤属植物的药用质量评价提供参考。方法 Unitary C18色谱柱(250mm×4.6 mm,5μm);流动相为乙腈(A)-0.2%磷酸水溶液(B);二元高压梯度洗脱;检测波长205 nm;体积流量1 m L/min;进样量20μL;柱温25℃。结果采用“中药色谱指纹图谱相似度评价软件(2004A)”标定了17份常春藤属样品的12个共有指纹峰,计算其相似度在0.715~0.992;通过聚类分析和主成分分析结果初步判断洋常春藤的综合药用质量要优于其他品种。结论建立的方法能够成功应用于筛选高质量的常春藤原料以及进行洋常春藤及其栽培品种药材的质量评价与控制,同时可为制定洋常春藤及其栽培品种药用质量评价研究提供新的参考依据。
OBJECTIVE: To establish a HPLC-UV fingerprint of saponins of Ivy and its cultivars, and to conduct statistical analysis by using cluster analysis and principal component analysis methods, combined with the determination of calibration compounds, Medical quality evaluation for reference. Methods Unitary C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile (A) -0.2% phosphoric acid solution (B). The binary elution was carried out at 205 nm with a flow rate of 1 mL / min. Injection volume 20μL; column temperature 25 ℃. Results The similarity of 12 common fingerprints of 17 samples of Ivy was calibrated using the “similarity evaluation software of chromatographic fingerprints of traditional Chinese medicine (2004A) ”, and the similarity between them was calculated from 0.715 to 0.992. By cluster analysis and principal component analysis The preliminary conclusion is that the comprehensive medicinal quality of Ivy is superior to other varieties. Conclusion The established method can be successfully applied in the screening of high-quality ivy raw materials as well as the quality evaluation and control of the medicinal materials of ivy and its cultivars, as well as to provide a new method for the evaluation of medicinal qualities of ivy and its cultivars Reference.