目的:探索茯苓菌液态发酵合成茯苓三萜的工艺条件。方法:从碳源、氮源、金属离子、辅助因子及初始pH角度对茯苓菌液态发酵合成茯苓三萜的过程进行系统分析,以确定高效发酵工艺。结果:最佳碳源为葡萄糖、氮源为玉米浆粉、添加金属离子ZnSO_4、MnSO_4、CuSO_4或FeSO_4以及辅助因子2-硫代巴比妥、初始pH范围为5~6。结论:通过培养基和发酵条件优化设计可显著提高茯苓三萜的合成效率。 Objective: To explore the technological conditions of Poria cocos Triterpenoids fermentation by Poria cocos culture. Methods: The process of liquid fermentation of Poria cocos by triterpenoids from carbon sources, nitrogen sources, metal ions, cofactors and initial pH was systematically analyzed to determine the efficient fermentation process. Results: The optimum carbon source was glucose and the nitrogen source was corn syrup. The metal ions such as ZnSO_4, MnSO_4, CuSO_4 or FeSO_4 and cofactor 2-thiobarbitol were added. The initial pH range was 5 ~ 6. Conclusion: The optimal design of culture medium and fermentation conditions can significantly improve the synthesis efficiency of triterpenoids.