本文从3个方面介绍了细菌转座子及其应用:①转座子载体的设计原理和目前广泛应用的细菌转座子载体。②近年来人工构建的细菌转座子,着重介绍了常用的工程转座子的特性以及在分子生物学研究中的用途。③转座子用于基因操作,包括细菌基因的体内、体外分子克隆和DNA核苷酸序列测定。还对已取得的成果作了介绍. In this paper, three aspects of bacterial transposon and its application: ① design principle of transposon vector and the currently widely used bacterial transposon vector. ② In recent years, artificial construction of transposable bacteria, focusing on the introduction of the characteristics of commonly used engineering transposons and molecular biology research purposes. ③ transposons for genetic manipulation, including bacterial genes in vitro and in vivo, molecular cloning and DNA sequencing. The achievements made have also been introduced.