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神经生长因子(NGF)是一种蛋白质,它能选择性地控制胚胎感觉神经细胞和交感神经细胞的生长、发育和修复。对它的生物学作用已作了广泛的研究,并有过详细的报导。用雄鼠下颌腺制备了2.5S NGF,并测定了其活性亚单位氨基酸的顺序。已证明,新生小鼠注射小量的抗NGF抗体,则引起大部分神经节的明显破坏,存活的神经节在大小上也有改变。由于NGF的正常来源不清,还缺乏有效的NGF特异性拮抗药物,故NGF的免疫学损伤仍然是评价NGF对神经系统发育生理作用的最好方法。本文用一系列抗小鼠2.5S NGF杂交瘤
Nerve growth factor (NGF) is a protein that selectively regulates the growth, development, and repair of embryonic sensory and sympathetic nerve cells. Its biological role has been extensively studied and has been reported in detail. 2.5S NGF was prepared from the male rat submandibular gland and the order of its active subunit amino acids was determined. It has been demonstrated that injection of a small amount of anti-NGF antibodies into neonatal mice causes significant destruction of most of the ganglia and that the surviving ganglia vary in size. Due to the unclear normal source of NGF and the lack of potent NGF-specific antagonists, the immunological damage of NGF is still the best way to evaluate the physiological role of NGF in the development of the nervous system. This article uses a series of anti-mouse 2.5S NGF hybridoma