利用同工酶谱研究稻种起源、亲缘远近、优势测定、生态型划分等已有很多报道。种、亚种、品种间的同工酶谱一般说来均有明显差异。一个基因、一个酶的观点巳得到广泛的承认。为了给研究杂交粳稻黎优57杂种优势问题、区别杂交种真伪提供科学依据,北京市种子公司与武汉大学遗传研究室协作,于1982年11月对杂交粳稻黎优67的同工酶谱进行了分析。试验方法:将黎优57及其三系和一些常规品种培育成二叶期的幼苗;取叶片研磨、离心沉淀、冰冻保存;采用聚丙烯酰胺凝胶电泳法对样品进行电泳,电泳完毕用醋酸α-萘酯与β-萘酯作染色底物,用坚牢兰 B 盐作显色剂 There are many reports about the origin, distantness and proximity of rice, the determination of its advantages, the division of ecotype and so on. Isoenzyme patterns between species, subspecies and varieties generally have significant differences. The idea of ​​a gene, an enzyme, has been widely recognized. In order to provide a scientific basis for distinguishing heterosis of hybrid japonica rice Liyou 57 from authenticity and falsehood of hybrids, Beijing Seed Company collaborated with Genetic Research Laboratory of Wuhan University in November 1982 to study isozyme patterns of hybrid rice Liyou 67 Analysis. Test methods: Liyou 57 and its three lines and some conventional varieties bred into two leaf stage seedlings; take leaves grinding, centrifugation, cryopreservation; using polyacrylamide gel electrophoresis samples were electrophoresis, electrophoresis was completed with acetic acid α-naphthyl ester and β-naphthyl ester as the dyeing substrate, with fast blue salt B as a developer