建立了噻·氟腈·苯醚悬浮种衣剂的高效液相色谱(HPLC)分析方法。采用C18色谱柱,以甲醇+水=90+10(V/V)为流动相,在220nm波长下,3种有效成分在8min内完全分离。噻虫嗪、氟虫腈和苯醚甲环唑的线性相关系数r分别为0.999 1、0.999 5、0.999 2,标准偏差为0.06%、0.02%、0.03%,变异系数为0.37%、0.79%、0.87%,平均回收率为99.77%、99.27%、99.33%。 A high performance liquid chromatography (HPLC) method was developed for the determination of thionoflurane and phenylacetamide suspended seed coating. Using C18 column with methanol + water = 90 + 10 (V / V) as the mobile phase, the three active components were completely separated within 8 min at 220 nm. The linear correlation coefficients of thiamethoxam, fipronil and difenoconazole were 0.999, 0.999 5 and 0.999 2, respectively. The standard deviations were 0.06%, 0.02% and 0.03%, and the coefficients of variation were 0.37% and 0.79% 0.87%, the average recovery was 99.77%, 99.27%, 99.33%.