霍乱毒素(CT)由A和B两个亚基组成,B亚基能和宿主细胞表面含有GM1的受体结合。作者曾报道小鼠T细胞与CT的结合力随鼠龄增长而降低。本文进一步发现人类T细胞也有类似情况。作者将15～80岁的健康志愿者按年龄分成7组,每组7～8人,男女数大致相等。每人取血10ml,用Ficoll-Hvpaque梯度离心分得淋巴细胞。再通过尼龙毛柱而得富含T Cholera toxin (CT) consists of two subunits, A and B, which bind to the receptor containing GM1 on the host cell surface. The authors have reported that mouse T cells and CT binding decreased with the growth of mice. This article further found that there are similar cases of human T cells. The authors divided healthy volunteers aged 15 to 80 by age into 7 groups of 7 to 8 people, roughly equal in number of men and women. Blood was collected from 10 ml each, and lymphocytes were separated by Ficoll-Hvpaque gradient centrifugation. And then through nylon wool column was rich in T