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黄天负(广西柳州市卫生防疫站):为了解镉中毒和镉吸收者的微环境的变化,对已确诊的三名镉中毒和六名镉吸收者进行外周血SCE检测,两组的作业工龄为12～21年,均为男性。以健康献血员作为对照组。 分别抽取受检者静脉血0.3ml轻轻注入1640培养基,避光培养24小时加入Brdu使其浓度为10μg/ml,于收获细胞前8小时加入秋水仙素0.6μg,72小时收获细胞,每瓶加0.075M氯化钾液4ml混匀置37℃低渗30分钟,常规制片,24小时后在50℃2×SSC液存在下用20W紫外线灯(相距4～
Huangtian negative (Guangxi Liuzhou City Health and Epidemic Prevention Station): In order to understand the cadmium poisoning and cadmium absorption of the microenvironment changes, has been diagnosed three cadmium poisoning and six cadmium absorbers peripheral blood SCE testing, two groups of operations Length of service 12 to 21 years, are men. Healthy blood donors as a control group. The venous blood of the subjects were drawn 0.3ml gently injected 1640 medium, incubated for 12 hours Brdu concentration of 10μg / ml, colchicine 0.6μg 8 hours before harvested cells, 72 hours harvest cells, each Bottle plus 0.075M potassium chloride solution 4ml mix 37 ℃ hypotonic for 30 minutes, conventional filming, 24 hours later in 2 × SSC liquid at 50 ℃ with 20W UV lamp (4 ~