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以魔芋属4个种的17份资源的花粉为试材,利用扫描电镜对其花粉进行形态观察,对观察结果进行形态分析和聚类分析.结果表明:(1)参试花粉属于大孢粉(50~100μm),其花粉壁上均无萌发孔或萌发沟,花粉形状为长球形或近球形,极面观为近圆形或扁圆形,白魔芋和西盟魔芋花粉外壁纹饰为条纹状,条纹间无穿孔,珠芽魔芋和疣柄魔芋外壁光滑或粗糙,其中缅甸珠芽A花粉外壁上出现了假沟.(2)缅甸珠芽A为单独一类,疣柄魔芋A、疣柄魔芋B和缅甸珠芽种子聚为一类,缅甸珠芽B和2010-052聚为一类,其余魔芋聚为一类,聚类结果与花粉外部形态表现的差异一致.(3)魔芋属种间花粉形状、花粉大小、纹饰细微特征、条脊粗细等方面存在明显差异,可为魔芋种间分类鉴定及魔芋属植物的系统演化提供重要依据.
The pollen morphology of 17 species of 4 species of Amorphophallus from Amorphophallus was studied by scanning electron microscopy (SEM), and morphological analysis and cluster analysis were conducted on the observed results.The results showed that: (1) (50 ~ 100μm), the pollen wall without germination holes or germination groove, the pollen shape is long spherical or nearly spherical polar surface is nearly round or oblate, white konjac and Amorphophallus pollen outer wall ornamentation stripes Shaped, fringe without perforation, kadsura konjac and warts handle konjac smooth or rough outer wall, in which Myanmar bud A pollen on the outer wall of the fossa. (2) Myanmar bud A is a separate category, warts handle Konjaku A, warts handle Konjaku B And Burmese pearl buds clustered into one category. Burma beads B and 2010-052 were clustered into one group, while the rest were clustered into one group. The clustering results were consistent with the pollen morphology. (3) Pollen size, fine features of ornamentation, and the thickness of the ridge were significantly different, which could provide an important basis for the classification and identification of konjac species and the systematic evolution of konjac.