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绿豆(Vigna radiata)在硫酸铜胁迫下水培发芽后再移植土种,观察胚芽和幼苗生长情况;测定胚芽和幼苗细胞内密度变化(DANCE反应),以及胚芽的超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量,研究萌发期硫酸铜胁迫对绿豆生长的影响及其DANCE反应。结果显示,硫酸铜胁迫抑制胚芽生长,以胁迫3~4 d最为显著,并出现芽细胞DANCE反应。移植土壤后,胁迫组幼苗出现代偿性加快生长,生长率随萌发期硫酸铜胁迫浓度增加而提高。土种3 d后,胁迫组幼苗表观生长状态恢复正常,但是,胁迫组幼苗根、茎、叶均出现DANCE反应。根、茎、叶部在土种1~2 d后均发生DANCE反应,其中根部反应持续1 d,茎部反应持续3 d,而叶部反应则持续至土种第5日。土种6 d后,各胁迫组幼苗均无DANCE反应。硫酸铜胁迫还导致胚芽SOD活性和MDA含量显著增高,SOD基因m RNA表达增加,提示硫酸铜胁迫促进胚芽产生自由基。研究结果表明,绿豆的DANCE反应可能是发芽减慢和幼苗代偿性生长所致,发芽期适当的环境胁迫可能有助于植物幼苗生长。
Mung bean (Vigna radiata) under copper sulfate stress after hydroponic germination and then transplanting soil to observe the growth of germ and seedling; determination of germ and seedling cell density changes (DANCE reaction), and germ superoxide dismutase (SOD) activity And malondialdehyde (MDA) content in the germinating period of copper sulfate stress on the growth of mung bean and its DANCE response. The results showed that copper sulfate inhibited the growth of embryo, which was the most obvious in 3 ~ 4 d of stress, and appeared DANCE response of bud cells. After transplanting the soil, the seedling of the stress group appeared compensatory and accelerated growth, and the growth rate increased with the concentration of copper sulfate stress during germination. After 3 d, the apparent growth status of the seedlings in the stress group returned to normal, however, the DANCE response was observed in the root, stem and leaf of the seedling in the stress group. The roots, stems and leaves all developed DANCE after 1 to 2 days of soil application, in which the root reaction continued for 1 day and the stem reaction continued for 3 days, while the leaf reaction lasted for 5 days. After 6 d of soils, there was no DANCE in seedlings of each stress group. Copper sulfate stress also resulted in significantly increased SOD activity and MDA content in germ and increased expression of m RNA in SOD gene, suggesting that copper sulfate stress can promote the generation of free radical in germ. The results showed that the DANCE response of mung bean may be caused by slow germination and compensatory growth of seedlings. Appropriate environmental stress during germination may contribute to plant seedling growth.