为确定产纳他霉素的褐黄孢链霉菌WZ-18的最佳发酵培养条件,研究了褐黄孢链霉菌菌株WZ-18产纳他霉素的发酵培养基成分及培养条件。结果表明:最佳培养基成分为葡萄糖40g/L、大豆蛋白胨20g/L、酵母粉7g/L、0.2%的磷酸镁,同时发酵48h时添加0.2%的丙酸钠;最佳培养条件为温度29℃、初始pH8.0、装液量40mL/300mL、接种量6%、转速200r/min;在此条件下培养,纳他霉素产量达到3.51g/L。 In order to determine the optimal fermentation conditions for producing natamycin-producing Streptomyces taurus WZ-18, the fermentation medium and fermentation conditions of natamycin-producing strain WZ-18 were studied. The results showed that the optimal culture medium consisted of 40g / L glucose, 20g / L soybean peptone, 7g / L yeast extract and 0.2% magnesium phosphate and 0.2% sodium propionate for 48h. The optimum culture conditions were temperature 29 ℃, initial pH8.0, liquid volume 40mL / 300mL, inoculum 6%, speed 200r / min; under this condition, natamycin production reached 3.51g / L.