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目的:探讨耐力运动和补充益气补肾中药对大鼠血清和睾丸睾酮水平以及骨骼肌AR mRNA表达的的影响。方法:50只Wistar大鼠随机分为安静对照组(n=10)、安静服药组(n=10)、训练组(n=15)和训练服药组(n=15)。经6周递增负荷游泳训练后,使用放免法测定大鼠睾丸和股四头肌匀浆总睾酮水平,利用RT-PCR方法检测大鼠深层股四头肌AR mRNA的表达水平。采用One-way Anova对组间数据进行比较,采用析因方差分析揭示训练和中药两种因素对所测指标的影响。结果:未服药训练大鼠的血清和睾丸组织睾酮水平明显下降(P<0.05);服用中药的运动大鼠血清和睾丸睾酮浓度未下降。训练对大鼠骨骼肌AR基因的表达有极显著的影响(p<0.001);中药同训练因素一样,亦能明显提高AR基因的表达水平,但是效应程度低于训练因素(p<0.01);两者的交互作用对AR基因表达的影响同样显著(p<0.01)。结论:长时间适应性训练和补充益气补肾中药可以促进增强大鼠股四头肌(Ⅱ型肌纤维为主)AR mRNA的表达。
Objective: To investigate the effects of endurance exercise and supplementing Qi and tonifying kidney on serum and testis levels in rats and the expression of AR mRNA in skeletal muscle. Methods: Fifty Wistar rats were randomly divided into the control group (n = 10), the sedentary group (n = 10), the training group (n = 15) and the training group (n = 15). After six weeks of incremental load swimming training, total testosterone in testis and quadriceps femoris was determined by radioimmunoassay. AR mRNA expression in deep quadriceps femoris was detected by RT-PCR. One-way Anova was used to compare the data between the two groups. The analysis of variance (ANOVA) revealed the influence of training and traditional Chinese medicine on the measured index. Results: The levels of testosterone in serum and testicular tissue were significantly decreased in non-medication rats (P <0.05). The testosterone levels in serum and testis of rats taking traditional Chinese medicine did not decrease. Training had a very significant effect on AR gene expression in skeletal muscle of rats (p <0.001). Chinese medicine, like training factors, also significantly increased the expression of AR gene, but the effect was lower than that of training factor (p <0.01). The effect of the interaction of the two on AR gene expression was also significant (p <0.01). CONCLUSION: Long-term adaptive training and replenishing of Qi-reinforcing and tonifying kidney herbs can promote the expression of AR mRNA in the quadriceps femoris muscle (type Ⅱ muscle fiber) in rats.