Objective:To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn.(A.E.),a Chinese medicinal herb,against coxsackievirus B3(CVB3).Methods:The antiviral effects of A.E.against CVB3 in vitro(primarily cultured myocardial cells) and in vivo(BALB/c mice) were determined. Serum pharmacological method was also adopted by in vitro experiments.The effects of A.E.inhibiting the CVB3 mRNA expression were compared by RT-PCR in mice in vivo.Results:A.E.exhibited obvious antiviral effects in vivo,and serum samples obtained from the rats with oral administration of A.E.(10μg/mL,5μg/mL) reduced the virus titers in the infected myocardial cells(3.00±0.70,3.55±0.52,P<0.01).Meanwhile,the viral myocarditis induced by CVB3 was inhibited significantly by A.E.,and the 15-day mortality was reduced to 40% and 45%(P<0.01) in mice treated with A.E.at doses of 50 mg/kg and 100 mg/kg,respectively,while the 30-day mortality was decreased to 45%and 50%,respectively(P<0.01).Moreover,the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E.Conclusion:Aqueous extract of Spatholobus suberectus Dunn.(A.E.) has inhibitory effect on CVB3 both in vitro and in vivo. Objective: To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. (AE), a Chinese medicinal herb, against coxsackievirus B3 (CVB3). Methods: The antiviral effects of AEagainst CVB3 in vitro (cultured rabbit cells) and Serum pharmacological method was also adopted by in vitro experiments. The effects of AE inhibition by the CVB3 mRNA expression were compared by RT-PCR in mice in vivo. Results: AEexhibited obvious antiviral effects in vivo and serum samples were obtained from the rats with oral administration of AE (10 μg / mL, 5 μg / mL) reduced the virus titers in the infected myocardial cells (3.00 ± 0.70, 3.55 ± 0.52, P <0.01) myocarditis induced by CVB3 was inhibited significantly by AE, and the 15-day mortality was reduced to 40% and 45% (P <0.01) in mice treated with AEat doses of 50 mg / kg and 100 mg / kg, respectively, while the 30-day mortality was decreased to 45% and 50%, respectively (P <0.01) .Mor eover, the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E.Conclusion: Aqueous extract of Spatholobus suberectus Dunn. (A.E.) has inhibitory effect on CVB3 both in vitro and in vivo.