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目的研究副溶血弧菌(VP)在不同盐分浓度下毒力表型的变化。方法利用小鼠致死性实验、兔回肠袢实验、Raw264细胞毒性实验和兔红细胞溶血活性实验比较标准强毒株RIMD2210633株在高盐(2%NaCl)和低盐(0.5%NaCl)条件下毒力表型的变化。结果 0.5%NaCl试验组副溶血弧菌的生长速度较2%NaCl试验组有所减慢,而产生的肠积液量、对Raw264细胞的细胞毒性及对兔红细胞的相对溶血活性却显著高于2%NaCl试验组(P<0.05);然而0.5%NaCl试验组菌体的小鼠致死率却低于2%NaCl试验组,两组小鼠存活率分别为80.0%和53.3%。结论在低盐条件下,VP的生长速度下降,肠毒性、Raw264细胞毒性和溶血活性均增强,而小鼠致死毒性反而减弱。
Objective To study the changes of virulence phenotypes of Vibrio parahaemolyticus (VP) under different salt concentrations. Methods The virulence of standard virulent strain RIMD2210633 in high salt (2% NaCl) and low salt (0.5% NaCl) strains was compared using lethal test in mice, ileocecal 兔experiment, Raw264 cytotoxicity assay and rabbit erythrocyte hemolysis test Phenotypic changes. Results The growth rate of Vibrio parahaemolyticus in 0.5% NaCl group was slower than that in 2% NaCl group. The intestinal fluid volume produced by 0.5% NaCl was significantly higher than that of Raw264 cells and the relative hemolysis to rabbit erythrocytes 2% NaCl group (P <0.05). However, the lethality of mice in 0.5% NaCl group was lower than that of 2% NaCl group. The survival rates of the two groups were 80.0% and 53.3%, respectively. Conclusions Under the condition of low salt, the growth rate of VP is decreased, the intestinal toxicity, Raw264 cytotoxicity and hemolytic activity are enhanced, but the lethal toxicity of mice is weakened.