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目的探讨广西肝癌高发区人群DNA修复基因8-羟基鸟嘌呤糖苷酶1单核苷酸多态性与肝细胞癌易感性的关系,并分析与环境危险因素的交互作用。方法采用以医院为基础的病例-对照研究,对广西壮族自治区500例肝细胞癌和507例正常对照进行流行病学调查,并应用TaqMan MGB荧光定量PCR方法检测hOGG1-Ser326Cys位点基因型。结果病例组和对照组中hOGG1-326Cys突变等位基因分布频率为24.60%和10.75%(P<0.05);该位点3种基因型Ser/Ser、Ser/Cys和Cys/Cys在病例组和对照组中分布频率分别为71.40%、8.00%、20.60%和84.22%、10.06%、5.72%(P<0.05);与携带hOGG1基因326位点野生纯合子Ser/Ser基因型者比较,携带至少一个hOGG1-326Cys突变等位基因的个体患肝癌风险显著增加(OR=2.14,95%CI:1.57～2.91);交互作用分析结果表明hOGG1基因326位点多态性与慢性HBV感染之间在肝细胞癌发生中具有交互作用,相乘模型交互作用指数为0.61,相加模型交互作用指数为1.31。结论 hOGG1-326Cys突变等位基因是肝细胞癌发生的危险等位基因;携带至少一个hOGG1-326Cys突变等位基因的个体若同时暴露于HBV慢性感染,患肝癌的风险显著增加。
Objective To investigate the association between DNA repair gene 8-hydroxyguanine glycosylase 1 (SNP) polymorphism and susceptibility to hepatocellular carcinoma in high incidence of liver cancer in Guangxi and its interaction with environmental risk factors. Methods A case-control study based on hospital was conducted in 500 cases of hepatocellular carcinoma in Guangxi Zhuang Autonomous Region and 507 normal controls. The genotypes of hOGG1-Ser326Cys were detected by TaqMan MGB real-time PCR. Results The frequency of allele distribution of hOGG1-326Cys in cases and controls was 24.60% and 10.75%, respectively (P <0.05). The frequencies of Ser / Ser, Ser / Cys and Cys / The frequency of distribution in the control group was 71.40%, 8.00%, 20.60% and 84.22%, 10.06% and 5.72% respectively (P <0.05). Compared with wild type homozygote Ser / Ser genotype 326 carrying hOGG1 gene, The risk of hepatocellular carcinoma was significantly increased in individuals with a mutation of hOGG1-326Cys (OR = 2.14, 95% CI: 1.57-2.91). The results of the interaction analysis indicated that there was a significant difference between the 326 polymorphism of hOGG1 gene and chronic HBV infection in liver There was an interaction in the occurrence of cell carcinoma, the multiplication model interaction index was 0.61, and the additive model interaction index was 1.31. CONCLUSION: The hOGG1-326Cys mutation allele is a risk allele of hepatocellular carcinoma. The risk of liver cancer is significantly increased in individuals with at least one hOGG1-326Cys mutation allele when they are simultaneously exposed to chronic HBV infection.