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目的:初步阐述缺氧诱导因子-1α(HIF-1α)及血管内皮生长因子(VEGF)在宫颈鳞癌组织中异常表达的可能机制及两者与各种临床病理因素的关系及其预后价值。方法:应用免疫组化S-P法和蛋白印迹免疫分析法测定HIF-1α及VEGF,所得数据应用SPSS11.0系统软件包进行处理。结果:免疫组化结果:HIF-1α和VEGF在宫颈组织中阳性表达率分别为62.07%和49.43%;HIF-1α和VEGF的表达随着临床分期的增高及病理分化的降低表达增强,两者在原位癌、早期浸润癌和晚期浸润癌中的表达差异有统计学意义,脉管内见癌栓者HIF-1α的表达增强,两者的表达与淋巴结转移情况无关。VEGF的表达与HIF-1α的表达呈正相关。蛋白印迹免疫分析显示:在120KD和23KD位置可见HIF-1α和VEGF的蛋白条带,早期宫颈鳞癌组织中HIF-1α蛋白表达量是正常宫颈组织中蛋白表达量的(5.1±1.62)倍,早期宫颈鳞癌组织中VEGF蛋白表达量是正常宫颈组织中蛋白表达量的(4.2±1.62)倍。结论:HIF-1α和VEGF的联合检测对判断宫颈鳞癌的病情和预后具有重要的意义。
Objective: To investigate the possible mechanism of abnormal expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in cervical squamous cell carcinoma and their relationship with various clinicopathological factors and their prognostic value. Methods: HIF-1α and VEGF were detected by immunohistochemical S-P method and Western blot immunoassay. The data were processed by SPSS11.0 system software package. Results: The positive rates of HIF-1α and VEGF in cervical tissues were 62.07% and 49.43% respectively. The expressions of HIF-1α and VEGF were increased with the increase of clinical stage and pathological differentiation, both The expression of HIF-1α in carcinoma in situ, early invasive carcinoma and advanced invasive carcinoma was significantly different, and the expression of HIF-1α in the tumor was enhanced in the vessel. There was no correlation between the expression of HIF-1α and the lymph node metastasis. The expression of VEGF was positively correlated with the expression of HIF-1α. Western blot immunoassay showed that the protein bands of HIF-1α and VEGF were detected at 120KD and 23KD. The expression of HIF-1α protein in early cervical squamous cell carcinoma was 5.1 ± 1.62 times that of normal cervical tissue, The expression of VEGF protein in early stage of cervical squamous cell carcinoma was (4.2 ± 1.62) times that of normal cervical tissue. Conclusion: The combined detection of HIF-1α and VEGF is of great significance in judging the condition and prognosis of cervical squamous cell carcinoma.