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Objective To explore the characteristics of Helicobacter pylori (H.pylori) antige n in serum and to evaluate its clinical diagnostic value. Methods Enzyme-linked immunosorbant assay (ELISA) was developed to detect the soluble H.pylori antigen (S-Hp) and circulatory specific H.pylori antigen imm unocomplexes (Hp-IC) in serum. Results The positive rate of S-Hp was 90.91% from 66 patients with H. pylo ri infection, which was much greater than 0% found in 28 controls ( P <0.0 01). Moreover, its concentration closely reflected the number of H.pylori in the gastric mucosa layer. We also found that Hp-IC existed bound with IgG a nd/or IgA in patients with positive S-Hp. However, there is no evidence to sho w the concentration of S-Hp reduced significantly in followed-up subjects afte r effective therapy. Conclusions These methods as newly and noninvasive complementary tools can be used for clini cal diagnosis of H.pylori infection. In addition, S-Hp and Hp-IC may be of importance in H.pylori pathogenesis.
Objective To explore the characteristics of Helicobacter pylori (H. pylori) antige n in serum and to evaluate its clinical diagnostic value. Methods Enzyme-linked immunosorbant assay (ELISA) was developed to detect the soluble H. pylori antigen (S-Hp) and Results The positive rate of S-Hp was 90.91% from 66 patients with H. pylo ri infection, which was much greater than 0% found in 28 controls (P <0.0 01). Moreover, its concentration closely reflected the number of H. pylori in the gastric mucosa layer. We also found that Hp-IC existed bound with IgG a nd / or IgA in patients with positive S-Hp. However, there is no evidence to sho w the concentration of S-Hp reduced significantly in followed-up subjects afte r effective therapy. Conclusions These methods as newly and noninvasive complementary tools can be used for clini cal diagnosis of H. pylori infection. -Hp and Hp-IC may be of importance in H. pylori pathogenesis.