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目的 观察阿司匹林和肿瘤坏死因子α(TNF α)对脂肪细胞糖代谢和胰岛素敏感性的影响。方法 检测阿司匹林和TNF α处理 3T3 L1脂肪细胞对培液中葡萄糖消耗量的影响 ,以 2 脱氧 3 H D 葡萄糖摄入法观察葡萄糖的转运率 ,用半定量RT PCR观察胰岛素信号系统转导相关基因的表达。结果葡萄糖消耗实验发现 ,5mmol/L阿司匹林和 5 μg/LTNF α作用 48h使 3T3 L1脂肪细胞葡萄糖的消耗分别增加 2 5 %和 46% ,在 10 0nmol/L胰岛素存在条件下 ,阿司匹林轻度增加葡萄糖消耗 ( 10 % ) ,而TNF α却显著减低葡萄糖的消耗 ,同时用阿司匹林和TNF α处理 ,脂肪细胞的葡萄糖消耗较单独TNF α处理明显增加 ;葡萄糖转运实验发现 ,5mmol/L的阿司匹林作用 72h增加 3T3 L1脂肪细胞基础和胰岛素刺激的葡萄糖转运 (P <0 .0 1) ,5 μg/L的TNF α作用 72h增加基础葡萄糖转运 (P <0 .0 5 ) ,但抑制胰岛素刺激的葡萄糖转运 ,同时加入阿司匹林后 ,减低TNF α对胰岛素刺激的葡萄糖转运的抑制 (P <0 .0 5或P <0 .0 1)。半定量RT PCR发现 ,TNF α抑制葡萄糖转运体 4(GLUT4)和c cbl相关蛋白 (CAP)基因的表达 ,而阿司匹林并不影响GLUT4和CAP的表达 ,但是能减低TNF α对GLUT4和CAP表达的抑制作用。结论 在 3T3 L1脂肪细胞 ,阿司匹林增加
Objective To observe the effects of aspirin and tumor necrosis factor α (TNF α) on the glucose metabolism and insulin sensitivity of adipocytes. Methods The effect of aspirin and TNFα on glucose consumption in 3T3 L1 adipocytes was detected. The glucose transport rate was measured by 2-deoxy-3 HD glucose uptake. The signal transduction-related genes of insulin signaling system expression. Results Glucose consumption experiment showed that 5mmol / L aspirin and 5μg / LTNF α for 48h increased the glucose consumption of 3T3 L1 adipocytes by 25% and 46%, respectively. In the presence of 100 nmol / L insulin, aspirin slightly increased glucose (10%), while TNFα significantly reduced glucose consumption, while the treatment with aspirin and TNFα significantly increased the glucose consumption of adipocytes compared with TNFα alone. Glucose transport assay showed that 5mmol / L aspirin increased 72h 3T3 L1 adipocytes and insulin-stimulated glucose transport (P <0.01), and 5 μg / L of TNFα increased basal glucose transport (P <0.05) at 72 h but inhibited insulin-stimulated glucose transport, The addition of aspirin at the same time reduced the inhibition of insulin-stimulated glucose transport by TNFα (P <0.05 or P <0.01). Semiquantitative RT-PCR revealed that TNFα inhibited the expression of GLUT4 and c cbl-related protein (CAP), while aspirin did not affect the expression of GLUT4 and CAP, but decreased the expression of GLUT4 and CAP Inhibition. Conclusions Aspirin increases in 3T3 L1 adipocytes