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本文利用液体闪烁测量~3H—TdR体外渗入小鼠肝癌腹水型细胞的技术观察胍唑(Guan-azole即3,5二氨基1,2,4三唑)等药物对该肿瘤细胞DNA合成的抑制作用。实验表明,胍唑0.25mg,0.5mg及1.0mg体外作用于1mL小鼠肝癌腹水型细胞悬液1.5小时后,明显抑制~3H—TDR的掺入,抑制率分别为77.57±8.69,91.95±4.18及96.80±2.20。其抑制程度随胍唑剂量加大而增强。与文献报导胍唑能抑制核苷酸还原酶,从而抑制
In this paper, the use of liquid scintillation measurement of ~ 3H-TdR infiltration of mouse hepatoma ascites cells in vitro technology observation guanidine (Guan-azole 3,5 diamino 1,2,4 triazole) and other drugs on the tumor cell DNA synthesis inhibition effect. Experiments show that guanazole 0.25mg, 0.5mg and 1.0mg in vitro 1mL mouse liver cancer ascites cell suspension after 1.5 hours, significantly inhibited the 3H-TDR incorporation, the inhibition rates were 77.57 ± 8.69,91.95 ± 4.18 And 96.80 ± 2.20. The inhibition increased with the dose of guanazole increased. And the literature reported that guanidine can inhibit nucleotide reductase, thereby inhibiting