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目的观察外源野生型 p5 3基因在肝癌基因治疗方面的可行性。方法将载有人野生型 p5 3- c DNA的真核表达质粒 p5 3- pc DNA3,用阳离子脂质体介导转染人肝癌细胞系 Hep G2 ,用流式细胞仪检测 p5 3- pc DNA3对 Hep G2细胞生长的影响。结果通过观察细胞生长曲线与流式细胞仪检测细胞周期和细胞的凋亡指数发现 ,Hep G2细胞生长受到明显的抑制。结论脂质体介导的 p5 3基因可在 Hep G2细胞中表达 ,且明显抑制该细胞的生长
Objective To observe the feasibility of exogenous wild-type p53 gene in gene therapy of liver cancer. Methods The human eukaryotic expression plasmid p53-pc DNA3 containing human wild-type p53-c DNA was transfected into human hepatocellular carcinoma cell line Hep G2 with cationic liposome, and the p5 3-pc DNA3 pair was detected by flow cytometry. Effect of Hep G2 cell growth. Results The growth of Hep G2 cells was significantly inhibited by observing the cell growth curve and flow cytometry to detect the cell cycle and cell apoptosis index. Conclusion The liposome-mediated p53 gene can be expressed in Hep G2 cells and significantly inhibits the growth of this cell.