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本文介绍用体外连续培养恶性疟原虫的方法建立新株的情况。作者用流灌法与蜡烛缸法培养成功新株FCR-2/CSA,FCR-3/FMG及FCR-4/6252。 FCR-2/CSA株采自一位旅游南美的青年人。1975年9月9日取病人血4毫升,在冰箱保存一夜后,于9月10日注射于一只未切脾的夜猴腹腔,10月15日此猴厚滴血片中每100个白细胞有28个疟原虫,取血冰冻后保存于液氮罐中。1976年4月1日将此冰
This article describes the establishment of new strains using a continuous culture of Plasmodium falciparum in vitro. The authors used successful flow-irrigation and candle-cylinder culture of new strains FCR-2 / CSA, FCR-3 / FMG and FCR-4/6252. The FCR-2 / CSA strain was collected from a young South American traveler. September 9, 1975 to take the patient’s blood 4 ml, stored overnight in the refrigerator on September 10 was injected into a peritoneal laparotomy of the night monkey, October 15, the thickness of the blood drop per 100 blood cells in the white blood cells There are 28 malaria parasite, blood and frozen in liquid nitrogen storage tank. April 1, 1976 this ice