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目的观察八味沉香胶囊对大鼠局灶性脑缺血的保护作用,初步探讨其作用机制。方法采用改良的线栓法制作大鼠大脑中动脉栓塞模型(MCAO),进行大鼠的神经行为学评分,测定丙二醛(MDA)水平、超氧化物歧化酶(SOD)活性,病理切片观察神经元形态结构的改变,免疫组织化学方法检测核因子-κB(NFκ-B)蛋白的表达。结果八味沉香胶囊能减少局灶性脑缺血大鼠的神经功能评分,降低局灶性脑缺血后血清中MDA水平,提高SOD活性,神经细胞形态的改变与模型组比较明显减轻,并且抑制NFκ-B的表达。结论八味沉香胶囊对大鼠局灶性脑缺血有明显的保护作用,其机制可能是通过有效地拮抗自由基损伤,提高脑组织抗氧化能力,抑制炎症与免疫反应来实现的。
Objective To observe the protective effect of Bawei Shenxiang Capsule on focal cerebral ischemia in rats and to explore its mechanism. Methods The rat model of middle cerebral artery occlusion (MCAO) was made by modified thread method. The neurobehavioral scores of rats were measured. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) The morphological changes of neurons were observed. The expression of nuclear factor-κB (NFκB) was detected by immunohistochemistry. Results Bawei Shenxiang Capsule can reduce the neurological deficit score in focal cerebral ischemia rats, decrease the level of MDA in serum after focal cerebral ischemia and increase the activity of SOD. The change of neuronal morphology is obviously reduced compared with model group Inhibition of NFκ-B expression. Conclusion Baweishenxiang capsule has a significant protective effect on focal cerebral ischemia in rats. The mechanism may be through antagonizing the free radical damage effectively, improving the antioxidant capacity of brain tissue and inhibiting inflammation and immune response.