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目的比较大黄蟅虫传统丸剂与超微粉剂对肾间质纤维化大鼠模型的药效作用。方法实验动物分为假手术组、模型组、传统丸剂组(0.54g/kg/d)、超微粉剂组(0.27g/kg/d)4组。采用单侧输尿管结扎的方法复制大鼠肾间质纤维化模型,用大黄蟅虫传统丸剂与超微粉剂进行干预,观察不同剂型对肾间质纤维化大鼠肾功能指标、血清纤维化指标、病理组织变化的影响。结果与假手术组相比,模型组血清肌酐(Cr)、尿素氮(Urea)、透明质酸(HA)、层黏蛋白(LN)、IV型胶原(IV-C)、Ⅲ型前胶原(PCIII)显著升高(P<0.01),肾间质纤维化明显。与模型组相比,大黄蟅虫传统丸剂组与超微粉剂组血清Cr、Urea、HA、LN、PCⅢ、IV-C降低(P<0.01),肾小管损害和肾间质纤维化程度减轻。与传统丸剂组相比,超微粉剂组血清Cr、Urea较低(P<0.05),其余指标差异无统计学意义。结论大黄蟅虫传统丸剂与超微粉剂对实验性肾间质纤维化都有较好的防治作用,能降低肾间质纤维化程度,改善肾功能;在改善肾功能方面,超微粉剂型优于传统丸剂型。复方“大黄蟅虫”药材超微粉碎后减少药量的同时有助于提高药效,有利于剂型现代化。
Objective To compare the pharmacodynamic effects of Rhubarb worm traditional pills and AMD powder on rats with renal interstitial fibrosis. Methods The experimental animals were divided into 4 groups: sham operation group, model group, conventional group (0.54g / kg / d) and superfine group (0.27g / kg / d) Renal interstitial fibrosis model was induced by unilateral ureteral ligation, and the traditional Chinese medicine pill and superfine powder were used to intervene to observe the effects of different dosage forms on renal function indexes, serum fibrosis indexes, The impact of pathological changes. Results Compared with the sham operation group, the levels of serum creatinine (Cr), urea (Urea), HA, LN, IV-C, PCIII) was significantly higher (P <0.01), renal interstitial fibrosis significantly. Compared with the model group, the levels of Cr, Urea, HA, LN, PCⅢ and IV-C in the Rhubarb worm group and the AMD group were significantly decreased (P <0.01), and the extent of tubule damage and renal interstitial fibrosis were alleviated. Compared with the traditional pill group, ultramicro powder group serum Cr, Urea lower (P <0.05), the remaining indicators no significant difference. Conclusion Rhubarb worm traditional pills and superfine powder on experimental renal interstitial fibrosis have a better prevention and treatment can reduce the degree of renal interstitial fibrosis and improve renal function; in improving renal function, superfine powder form excellent In the traditional pill type. Compound “Rhubarb worm” after ultrafine crushing medicine to reduce the dose at the same time help to improve efficacy, is conducive to the modernization of dosage forms.