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为探讨DREBs在果实发育及成熟调控中的作用及机理,以番茄‘Micro-Tom’为试材,克隆得到1个DREBs转录因子SlDREB1B。研究证实,在果实中SlDREB1B的表达可受脱水胁迫和温度胁迫诱导。将SlDREB1B构建到带有e-GFP报告基因的载体上,采用叶盘法将SlDREB1B基因转入番茄,经报告基因筛选和SlDREB1B表达检测,成功获得转基因阳性植株。和转空载基因的植株相比,转SlDREB1B植株的果实发育和成熟进程明显加速。进一步分析表明,SlDREB1B的过量表达,可调控一系列与果实发育和成熟相关的基因表达,其中,色素合成相关基因和果实软化相关基因的表达受到强烈诱导,说明SlDREB1B可通过调节果实成熟相关基因的表达影响番茄果实发育和成熟进程。
In order to investigate the function and mechanism of DREBs in fruit development and maturation, a DREBs transcription factor, SlDREB1B, was cloned using ’Micro-Tom’ from tomato. Studies have shown that the expression of SlDREB1B in fruit can be induced by dehydration and temperature stress. SlDREB1B was constructed on a vector carrying e-GFP reporter gene. SlDREB1B gene was transferred into tomato by leaf disc method. Transgenic positive plants were successfully obtained by screening reporter gene and detecting S1DREB1B expression. Compared with the plants transformed with empty vector, the development and maturation of fruits in S1DREB1B plants accelerated significantly. Further analysis showed that overexpression of SlDREB1B regulated a series of gene expression related to fruit development and maturation, in which expression of genes related to pigment synthesis and fruit softening were strongly induced, indicating that SlDREB1B can regulate the expression of genes related to fruit ripening Expression affects tomato fruit development and maturation process.