论文部分内容阅读
目的制备5-氟尿嘧啶(5-FU)有机金属框架(BTC)纳米粒,考察体外细胞摄取率及其影响因素。方法用室温配位调控法制备BTC纳米粒5-FU-Cu-BTC,用扫描电子显微镜观察其形态,用流式细胞仪测定其体外细胞摄取率及影响因素。结果 Cu-BTC载体的粒径约为100 nm,载药量为38%,体外细胞摄取实验表明:细胞摄取率与样品浓度和温度成正比。结论有机金属框架纳米粒可载入抗肿瘤药物并被肿瘤细胞摄取。
OBJECTIVE To prepare 5-fluorouracil (5-FU) organometallic framework (BTC) nanoparticles to investigate the uptake rate of cells in vitro and its influencing factors. Methods BTC nanoparticles 5-FU-Cu-BTC was prepared by coordination at room temperature. The morphologies of the BTC nanoparticles were observed by scanning electron microscopy. The cell uptake rate and its influencing factors were determined by flow cytometry. Results The particle size of Cu-BTC was about 100 nm and the drug loading was 38%. In vitro cell uptake experiments showed that the cell uptake rate was proportional to the sample concentration and temperature. Conclusions Organometallic framework nanoparticles can be loaded with antitumor drugs and taken up by tumor cells.