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利用C18柱对乙醇提取的芦荟浸膏进行分离纯化,得到蒽醌单体,核兹共振分析确证单体的化学结构,高压液相色谱分析单体的纯度;结果表明从芦荟的乙醇提取物中分离得到5个单体,分别为芦荟大黄素、羟基芦荟大黄素、原儿茶酸、芦荟色苷G、8-C-glu-7-O-methyl-(S)-Aloesol,其纯度分别为98.55%、99.22%、98.51%、98.76%、98.12%。另外,采用DPPH方法对5个成分进行抗氧化测试,同时与TBHQ、维生素C和维生素E进行了对比实验。结果表明其中大黄素、原儿茶酸和芦荟色苷G等3个化合物具有抗氧化性,而芦荟大黄素和8-C-glu-7-O-methyl-(S)-Aloesol没显示抗氧化性,该实验结果为芦荟的进一步开发利用提供佐证。
The C18 column was used to separate and purify aloe extract from ethanol to obtain anthraquinone monomer. The chemical structure of the monomer was confirmed by nuclear resonance analysis. The purity of the monomer was analyzed by high pressure liquid chromatography. The results showed that from the ethanol extract of aloe, Five monomers were isolated, which were aloe-emodin, aloe-emodin, protocatechuic acid, aloe-glycoside G and 8-C-glu-7-O-methyl- 98.55%, 99.22%, 98.51%, 98.76%, 98.12%. In addition, five components were tested for antioxidant activity using the DPPH method, and comparative experiments were conducted with TBHQ, vitamin C and vitamin E. The results showed that three compounds such as emodin, protocatechuic acid and aloe-glycoside G had antioxidant activity, whereas aloe-emodin and 8-C-glu-7-O-methyl- The results of this experiment provide further evidence for the further development and utilization of aloe vera.