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MicroRNA(miRNA)可用于癌症的早期诊断、预后判断,其分析检测具有重要临床意义.结直肠癌的发生、发展与miRNA 21、miRNA 92等的异常表达明显相关.本研究设计了以poly(d T)n为引导链的DNA探针(probe)并尝试使用α-溶血素(α-HL)纳米孔道单分子检测方法检测结直肠癌miRNAs.miRNA·probe复合物分子穿过α-HL纳米孔道限域空间时,由于probe链长、序列不同导致probe-α-HL相互作用不同,miRNA 92·probe 92、miRNA 21·probe 21、miRNA 16·probe 16输出为形态、阻断时间不同的多台阶特征信号,实现了三种miRNAs的有效区分.实验证明,此方法可以用于检测血清实际样品.因此,未来有望使用α-HL构建miRNA超灵敏单分子生物传感器.
MicroRNA (miRNA) can be used in the early diagnosis and prognosis of cancer, and its analysis has important clinical significance.The occurrence and development of colorectal cancer are closely related to the abnormal expression of miRNA 21 and miRNA 92. In this study, T) n is the DNA probe of the guide strand and attempts to detect the colorectal cancer miRNAs. MiRNA probe complex molecule through the α-HL nanopore (α-HL) nanopore single molecule assay Due to the different probe-α-HL interactions due to the different probe sequences, miRNA 92 · probe 92, miRNA 21 · probe 21, miRNA 16 · probe 16 can be output in multiple stages with different morphological and blocking times Which can effectively distinguish three kinds of miRNAs.Experiments show that this method can be used to detect the actual samples of serum.Therefore, it is expected to use α-HL in the future to construct miRNA ultrasensitive monomolecular biosensors.