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基于微流控芯片-激光诱导荧光分析技术,探讨了奶酪中两种β-酪啡肽(β-CM-5和β-CM-7)的场放大进样富集和检测.采用10 mmol/L硼酸-硼砂溶液(pH8.7)作为衍生缓冲液,5 mmol/L硼砂溶液(pH8.9)作为样品缓冲液,进样10s,60 mmol/L硼砂溶液(pH8.9)作为运行缓冲液,分离电压1500V,在120s内成功分离检测了两种 β-酪啡肽.实验结果表明,β-CM-5和β-CM-7的富集倍数是16倍和21倍,检出限分别是8.2 nmol/L和3.6 nmol/L,线性范围分别是0.05～2μmol/L和0.02～1μmol/L,加标回收率在86.9％ ～107.5％,该方法可应用于奶制品中β-CM-5和β-CM-7的含量测定.“,”A rapid and sensitive method was established for the separation and determination of β-casomorphins, including β - CM-5 and β - CM-7, by microchip capillary electrophoresis ( MCE ) coupled with laser induced fluorescence ( LIF ) detector based on field amplified sample stacking (FASS). The β-CMs were derivatized with 10 mmol/L boric acid-borate buffer solution (pH8. 7). The samples were diluted with 5 mmol/L borate buffer solution ( pH8. 9 ) and the injection time was 10s. The running buffer was 60 mmol/L borate solution ( pH8. 9 ) and the separation voltage was 1500V. β-CM-5 and β - CM-7 were successfully separated and detected within 120s, and enhancement factors of 16-and 21-fold were obtained. The limits of detection were 8. 2 nmol/L and 3. 6 nmol/L, with spiked recoveries of 86. 9％ ～107. 5％. The linear ranges were 0. 05 ～2 μmol/L and 0. 02～1μmol/L, respectively. The method could be applied to detectβ-CM-5 andβ-CM-7 in dairy foods, which are significant to the study on biologically active peptides in foods.