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作者从患自发性乳腺癌的BALB/c小鼠体内,取出实体肿瘤,用等渗盐水制成细胞悬液。以200μl癌细胞悬液注入5只雌性BALB/c小鼠背部,诱导出实体癌,建立了一株称为JC的肿瘤细胞系,该细胞系保留了腺癌的特征及在BALB/c小鼠体内产生肿瘤的能力。作者报告用与JC细胞系有交叉反应的抗培养的人乳腺癌细胞的小鼠单克隆抗
The authors removed solid tumors from BALB/c mice with spontaneous breast cancer and made cell suspensions with isotonic saline. After injecting 200 μl of a cancer cell suspension into the back of 5 female BALB/c mice to induce solid cancer, a tumor cell line called JC was established. This cell line retained the characteristics of adenocarcinoma and was used in BALB/c mice. The ability to produce tumors in the body. The authors report mouse monoclonal antibodies against human breast cancer cells that cross-react with JC cell lines