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金仓鼠肺细胞的恶性转化系统,由于其敏感、自发转化率低,早在1972年已见报道。李申德等首先在国内利用这个系统进行了细胞培养中癌变原理的研究。但此法的缺点在于肺细胞的分离比较困难,存活率很低。根据文献报道的方法分离细胞,一胎乳鼠(6—12只),只能得到1—2中瓶(培养面积28cm~2,培养瓶容积75ml),从而影响了这个恶性转化系统的推广。因此,我们试探改变分离金仓鼠肺细胞的条件并加以比较,探索使肺细胞存活率增高的方法。
The malignant transformation system of golden hamster lung cells has been reported as early as 1972 due to its low sensitivity and spontaneous conversion rate. Li Shende and others first used this system to study the principle of canceration in cell culture. However, the disadvantage of this method is that the separation of lung cells is difficult and the survival rate is very low. According to the method reported in the literature, the cells were isolated and only one to two suckling mice (6-12) were able to obtain 1-2 vials (culture area 28 cm-2, culture flask volume 75 ml), which affected the promotion of this malignant transformation system. Therefore, we tried to change the conditions of the separation of the golden hamster lung cells and compare them, and explore ways to increase the survival rate of lung cells.