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用免疫组化方法观察胰岛素样生长因子Ⅱ（ＩＧＦ－Ⅱ）、ＨＢｘＡｇ及ｒａｓ基因的表达蛋白ｐ２１在乙肝病毒（ＨＢＶ）和黄曲霉素Ｂ１（ＡＦＢ１）致树鼠句肝癌过程中的表达。发现这三种基因蛋白的表达与肝癌发生呈正相关系。即接受ＨＢＶ和ＡＦＢ１双因素的树鼠句的肝癌发生率与ＩＧＦ－Ⅱ、ＨＢｘＡｇ及ｐ２１的表达阳性率均显著高于只暴露于单一因素ＨＢＶ或ＡＦＢ１的动物。同时，在带瘤树鼠句中，这三种基因的表达又显著高于无瘤者。提示：ＨＢＶ与ＡＦＢ１能协同激发这些基因的异常表达；这些基因的过量表达在肝细胞的癌变过程中起了重要的作用
Immunohistochemistry was used to observe the expression of insulin-like growth factor II (IGF-II), HBxAg and ras gene expression protein p21 in hepatocarcinogenesis induced by hepatitis B virus (HBV) and aflatoxin B1 (AFB1). It was found that the expression of these three gene proteins is in a positive relationship with the occurrence of liver cancer. The incidence of hepatocellular carcinoma and IGF-II, HBxAg, and p21 expression were significantly higher in mice and rats that received both HBV and AFB1 than those animals exposed to a single factor, HBV or AFB1. At the same time, the expression of these three genes was significantly higher in tumor-bearing mice than in those without tumors. Tip: HBV and AFB1 can synergistically stimulate the abnormal expression of these genes; overexpression of these genes plays an important role in the carcinogenesis of hepatocytes.