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Objective: To investigate the distribution and clonality of TCR Vα subfamily T cells in patients with acute promyelocytic leukemia (APL). Methods: The complementary determining region 3 (CDR3) of TCR Vα 29 subfamily genes in peripheral blood mononuclear cells from 9 APL patients were amplified using RT-PCR. The positive products were further analyzed to identify the clonality of T cells by GeneScan technique. Results: One to seven of TCR Vα subfamilies could be detected in peripheral blood T cells from 9 cases with APL, the frequent expression of Vα subfamilies predominated in Vα3 and Vα19. Clonal expanded T cells could be detected in 8 APL patients, which predominant used Vα3, Vα26 or Vα27 (3 out of 8 cases). However, almost all Vα subfamilies with polyclonal expansion could be detected in peripheral blood T cells from 10 cases of normal individuals. Conclusion: Remarkable skew distribution and clonal expansion of TCR Vα subfamilies T cells is the common feature in patients with APL. Clonal expansion of T cells might reflect a response in host to APL cell as- sociated antigen, whether these expanded T cells have the ability for specific cytotoxicity against APL cells, remains an open question.
Objective: To investigate the distribution and clonality of TCR Vα subfamily T cells in patients with acute promyelocytic leukemia (APL). Methods: The complement region 3 (CDR3) of TCR Vα 29 subfamily genes in peripheral blood mononuclear cells from 9 APL patients were The positive products were further analyzed to identify the clonality of T cells by GeneScan technique. Results: One to seven of TCR Vα subfamilies could be detected in peripheral blood T cells from 9 cases with APL, the frequent expression of Vα subfamilies predominated in Vα3 and Vα19. Clonal expanded T cells could be detected in 8 APL patients, which predominant used Vα3, Vα26 or Vα27 (3 out of 8 cases). However, almost all Vα subfamilies with polyclonal expansion could be detected in peripheral Blood T cells from 10 cases of normal individuals. Conclusion: Remarkable skew distribution and clonal expansion of TCR Vα subfamilies T cells are the common feature in patients with APL. Clonal expansion of T cells might reflect a response in host to APL cell as-sociated antigen, whether these expanded T cells have the ability for specific cytotoxicity against APL cells, remains an open question.