论文部分内容阅读
90年代初,美国航空航天局(NASA)设计研制出一种转壁式生物反应器(Rotating Wall Vessel Bioreactor,RWVB)。采用RWVB进行基地试验时,意外地发现离体细胞在RWVB中呈现高密度聚集,并形成较大的组织样结构。RWVB的核心结构是由两个同心圆柱体构成的旋转培养装置。将细胞与培养液置入内、外圆柱体之间,整个装置绕水平纵轴旋转,使培养物长时间保持悬浮状态。由于在旋转过程中
In the early 1990s, NASA designed a Rotating Wall Vessel Bioreactor (RWVB). When using RWVB for base testing, it was unexpectedly found that ex vivo cells accumulated high density in RWVB and formed larger tissue-like structures. The core structure of RWVB is a rotating culture device composed of two concentric cylinders. The cells and culture solution into the inner and outer cylinders, the entire device about the horizontal axis of rotation, so that cultures remain in suspension for a long time. Due to the rotation process